Muzahar, Muzahar and Muhammad, Zairin and M. Agus, Suprayudi (2022) The phenotype comparison and genotype analysis of five Indonesian Laevistrombus sp. variants as a basis of species selection for aquaculture. AACL Bioflux, 11 (4). pp. 1164-1172.

Text THE PENOTYPE COMPARISON.pdf Download (412kB)

Abstract

Five morphological variants of Indonesian Laevistrombus sp. were successfully identified from Madong-Tanjungpinang marine waters. At first glance there are similarities in the collection of the conch, but when carefully observed there is a morphometric difference among the five. The condition had caused taxonomic uncertainty. For the purpose of selecting cultivation species, it was necessary to identify precisely by combining morphometric and genotype analyses. This study aimed to identify the species of Indonesian Laevistrombus sp. from Madong-Tanjungpinang city based on morphometrics and DNA sequence. The study was conducted in September-November 2017. Sampling collection was done along Madong waters. Traditional morphometrics was measured and was analysed by discriminant function analysis (DFA). Genotype analysis was conducted with specific primers of histone H3 gene. The DFA results showed significant morphometric differences in the five Laevistrombus groups, especially the thickness of outer shell lips. However, based on all morphometric and other physical characteristics, the five variants were one species of Laevistrombus turturella. The result was supported by a phylogenetic tree from DNA sequences of histone H3 gene that showed five variants having same histone H3 sequence. But, the sequences were different with Strombus canarium, S. vittatus, and S. epidromis. Histone H3 sequences of Laevistrombus turturella. had been submitted in the gene bank (submission ID: 2097081).

Actions (login required)

View Item